| YELLOWJERSEY | This was first used in the Tour de France in 1919, sixteen years after the first tour |
| BICYCLE | Human-powered vehicle used in the Tour de France, e.g. (7) |
| MILTON | English poet who composed his epic Paradise Lost, beginning in medias res, some 16 years after he had become completely blind (6) |
| LORRAINE | Former province of France ceded to Germany in 1871 after the Franco-Prussian war and regained by France in 1919 |
| ALSACE | Region of north-east France (3,200 sq miles; pop 2 million), ceded back to France in 1919 (6) |
| SHONA | Actress who's leaving her role as EastEnders' Whitney Dean after sixteen years (5,7) |
| MCGARTY | Actress who's leaving her role as EastEnders' Whitney Dean after sixteen years (5,7) |
| TWENTYTWENTY | Such perfect vision in sixteen years' time? |
| XAVIER | Sixteen years, off and on, protecting a Catholic missionary |
| TORNADO | Former RAF combat aircraft that was first used in the Gulf War in 1991 (7) |
| BRUSSELS | Starting location of the 2019 Tour de France chosen in honor of the 50th anniversary of Eddy Merckx's first Tour de France win |
| PINOT | Thibaut ____, whose surname is a grape variety, finished third in his first Tour de France in 2014 |
| WIGGINS | Who became the first Briton to win the Tour de France in 2012? (7) |
| HUGOKOBLET | Swiss cyclist who won the Giro d'Italia in 1950 and the Tour de France in 1951 |
| ACIDRAIN | Which phrase was first used in 1852 by Scottish chemist Robert Angus Smith, who was studying the chemistry of the atmosphere? (4,4) |
| LYEST | Finish line for the first stage of the first Tour de France |
| LYON | Finish line for the first stage of the first Tour de France |
| SUPERBS | Modern-day Skodas whose name was first used in the 1930s (7) |
| CHRISFROOME | Kenyan-born British cyclist who first won the Tour de France in 2013 (5,6) |
| LANCEARMSTRONG | Disgraced cyclist who won the Tour de France in a record-breaking seven consecutive years |
