| DOUBLEHELIX | Structure of this puzzle's theme, hidden in five answers |
| URL | Web address that can end with any of the suffixes hidden in five answers in this puzzle |
| FINDINGNEMO | 2003 animated hit, and activity that reveals puzzle theme hidden in 17-, 26-, 39- and 54-Across |
| HOUSEOFCARDS | Hit Netflix show that hints at the theme hidden in the puzzle's borders |
| HAM | With "on" and 59-Across, a hint to the theme hidden in three places in this puzzle |
| SUB | Type of vessel that went missing in June 2023 ... and is missing in five answers in this puzzle |
| SNAKE | Type of critter in five answers in this puzzle |
| INSIDEINFO | Tip, and a hint to this puzzle's theme found in five answers |
| REPEAT | What three-letter words do in five answers in this puzzle |
| GNU | African antelope "caged" in five answers in this puzzle |
| OWL | Hooter hiding in five answers of this puzzle |
| REPRISES | Repeated musical passages, and what happens in five answers in this puzzle |
| ROAD | Barricade, or something present in five answers (including this one) |
| LETDOWN | Bummer - and a hint to what's found in five answers |
| VIVA | Four in five answer test (4) |
| NIT | One of the "pickings" hidden in five puzzle answers |
| INDIGO | 1905 Nobel winner Adolf von Baeyer derived the molecular structure of this distinctive blue dye extracted from a plant of the same name, which is used in textiles and printing |
| SKI | Item hidden in five other answers |
| DNASEQUENCE | Human fingerprint, and what's hidden in five puzzle answers |
| TEST | Class ordeal hidden in five Across answers |
