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20 answers for: Natural identifier of two pens: the first one has ...
RANKANSWERCLUE
BIOMARKERNatural identifier of two pens: the first one has run out (9)
PENCILSHARPENERWorking with two pens, Charlie's right - this is no use to him (6,9)
EFFORTFULDetermined one has run out of truffle for cooking? (9)
DEARWith 67-Across, one of two pen names punnily hinted at by 20-, 37- and 52-Across (can you find the other one?)
LASTGASPAt the eleventh hour, when one has run out of pants? (4-4)
CARPEDIEMVehicle allowance has run out, so make the most of today
COGITABLEDog has run out with programme? That's rarely conceivable! (9)
RIGMAROLEFuss made by engineer before he has run out (9)
SAFEGUARDDefend crazy fears a drug has run out (9)
TENNESSEEState has run out of notes, behold!
PORTENDEDWarned drink has run out. (9)
FREEAGENTOne whose contract has run out
TELEPHONENothing stops Le Pen, the devious dog! (9)
ENCLOSUREPen the note, perhaps, that goes inside (9)
TESTAMENTTime after trial to pen the last word in witness statement (9)
CALLSIGNSIdentifiers of radio stations
SWANNERYColony of breeding/nesting cobs and pens, the birds associated with the annual tradition of "upping" (8)
ERRLast one has run right on to make a mistake
ELECTROThe one chosen has run out of plate (7)
DOEA female rabbit has run out of money, by the sound of it