| BIOMARKER | Natural identifier of two pens: the first one has run out (9) |
| PENCILSHARPENER | Working with two pens, Charlie's right - this is no use to him (6,9) |
| EFFORTFUL | Determined one has run out of truffle for cooking? (9) |
| DEAR | With 67-Across, one of two pen names punnily hinted at by 20-, 37- and 52-Across (can you find the other one?) |
| LASTGASP | At the eleventh hour, when one has run out of pants? (4-4) |
| CARPEDIEM | Vehicle allowance has run out, so make the most of today |
| COGITABLE | Dog has run out with programme? That's rarely conceivable! (9) |
| RIGMAROLE | Fuss made by engineer before he has run out (9) |
| SAFEGUARD | Defend crazy fears a drug has run out (9) |
| TENNESSEE | State has run out of notes, behold! |
| PORTENDED | Warned drink has run out. (9) |
| FREEAGENT | One whose contract has run out |
| TELEPHONE | Nothing stops Le Pen, the devious dog! (9) |
| ENCLOSURE | Pen the note, perhaps, that goes inside (9) |
| TESTAMENT | Time after trial to pen the last word in witness statement (9) |
| CALLSIGNS | Identifiers of radio stations |
| SWANNERY | Colony of breeding/nesting cobs and pens, the birds associated with the annual tradition of "upping" (8) |
| ERR | Last one has run right on to make a mistake |
| ELECTRO | The one chosen has run out of plate (7) |
| DOE | A female rabbit has run out of money, by the sound of it |