| ASHBYDELAZOUCH | Mary Queen of Scots was imprisoned in this Leicestershire castle in 1569 (5-2-2-5) |
| LEVEN | Loch near Kinross with a now ruined castle where Mary, Queen of Scots was imprisoned in 1567 (5) |
| TUSCANY | Cosimo I de' Medici became the first grand duke of this region in 1569 (7) |
| LOCH | Expanse of water in Scotland with seven islands, on one of which Mary Queen of Scots was imprisoned (4,5) |
| LOCHLEVEN | Castle in which Mary Queen of Scots was imprisoned (4,5) |
| FOTHERINGHAY | ___ Castle, fortress in Northamptonshire where Mary, Queen of Scots was executed in 1587 (12) |
| ENTOMBED | Mary Queen of Scots was ---- in Westminster Abbey in 1612 (8) |
| AXED | Imposed a duty to be executed as Mary Queen of Scots was (4) |
| COUSIN | Mary Queen of Scots was Elizabeth I's (6) |
| BADER | WWII flying ace, Douglas, who was imprisoned in Colditz Castle (5) |
| JOLIET | Illinois city where Jake was imprisoned in "The Blues Brothers" |
| MEDICI | Cosimo de' -; first grand duke of Tuscany from 1569 until his death in 1574 (6) |
| LITHUANIA | Republic of NE Europe, formerly federated with Poland by the Union of Lublin (1569) (9) |
| EDWARDV | Which king of England was imprisoned with his brother in the Tower of London and never seen again? (6,1) |
| KRAKOW | Port on the River Vistula; capital of Poland from 1038 to 1569 (6) |
| RUSSELL | Which Nobel Laureate was one of the founders of analytical philosophy and as an anti-war activist was imprisoned during the First World War? (7) |
| ROBBEN | ___ Island, site in Table Bay north of Cape Town where Nelson Mandela was imprisoned (6) |
| CARISBROOKECASTLE | Isle of Wight C12th fortification where Charles 1 was imprisoned for a year in 1647-8 (11,6) |
| BRUEGEL | Peter -------, Flemish painter of landscapes (1525-1569) (alt spelling) (7) |
| TIMOR | Where James Bond's boss was imprisoned by a crazy trio (5) |