| DARNLEY | Lord -; Mary, Queen of Scots' second husband Henry Stuart (7) |
| CARBERRY | ---- Hill, Mary Queen of Scots' second-last battle (8) |
| ELEANOR | Queen whose failed revolt against her husband, Henry II, resulted in her nigh on 16-year-long incarceration (7) |
| ANTONIA | ---- Fraser, biographer of Mary Queen of Scots (7) |
| SAOIRSE | ___ Ronan, star of the 2018 film Mary Queen of Scots (7) |
| SQUIRES | Members of a royal house including James I, Mary, Queen of Scots and Anne (7) |
| JAMESVI | Only child of Mary, Queen of Scots |
| COUSINS | E.g. Elizabeth I and Mary, Queen of Scots (7) |
| PARR | Queen consort of England who, following the death of her husband Henry VIII, married Thomas Seymour and retreated to Sudeley Castle accompanied by Lady Jane Grey (4) |
| BOLEYN | Earl of Wiltshire's daughter whose betrothal to Henry Percy was ended by Cardinal Wolsey on the orders of her future husband, Henry VIII (6) |
| RIDOLFI | --- plot, 1571 plot to assassinate Elizabeth I and replace her with Mary, Queen of Scots (7) |
| THECHOP | About to be dumped, like Mary Queen of Scots at Fotheringhay (3,3,3,4) |
| STUART | Henry -, Lord Darnley; second husband of Mary, Queen of Scots and the father of James I and VI of Scotland (6) |
| GUISE | Mary of ---, queen consort of James V of Scotland and mother of Mary, Queen of Scots (5) |
| MARY | --- of Guise, queen consort of James V of Scotland and mother of Mary, Queen of Scots (4) |
| BOTHWELL | 4th Earl of -; title Mary, Queen of Scots husband who was implicated in the murder of Lord Darnley (8) |
| LEVEN | Loch ___, body of water in Perth and Kinross, Scotland housing a castle that was a place of imprisonment of Mary, Queen of Scots (5) |
| ELIZABETHI | Henry VIII and Anne Boleyn's daughter whose reign as the last Tudor monarch saw the defeat of the Spanish Armada and execution of Mary, Queen of Scots (9,1) |
| WALSINGHAM | Surname of Queen Elizabeth I's spymaster who secured the execution of Mary Queen of Scots (10) |
| EARLOFBOTHWELL | Third husband of Mary, Queen of Scots, d. 1578 in Denmark (4,2,8) |
