| RIZZIO | Italian secretary of Mary Queen of Scots (6) |
| DAVIDRIZZIO | 16th-century Italian courtier who was murdered in 1566; the private secretary of Mary, Queen of Scots (5,6) |
| CARR | "The Italian Secretary" author Caleb |
| STUART | Henry -, Lord Darnley; second husband of Mary, Queen of Scots and the father of James I and VI of Scotland (6) |
| JAMESI | King of Scotland, son of Mary Queen of Scots (5,1) |
| FRASER | Antonia ---, biographer of Mary, Queen of Scots and Marie Antoinette |
| GUISE | Mary of ---, queen consort of James V of Scotland and mother of Mary, Queen of Scots (5) |
| MARY | --- of Guise, queen consort of James V of Scotland and mother of Mary, Queen of Scots (4) |
| DARNLEY | Lord -; title of Henry Stuart, husband of Mary, Queen of Scots implicated in the murder of David Rizzio before being murdered himself at Kirk o' Field (7) |
| LEVEN | Loch ___, body of water in Perth and Kinross, Scotland housing a castle that was a place of imprisonment of Mary, Queen of Scots (5) |
| WALSINGHAM | Surname of Queen Elizabeth I's spymaster who secured the execution of Mary Queen of Scots (10) |
| BOTHWELL | Earl of ---, third husband of Mary Queen of Scots (8) |
| ANTOINETTE | ___ of Bourbon, grandmother of Mary, Queen of Scots |
| LOCHLEVEN | Body of water in Scotland housing a castle that was the place of imprisonment of Mary, Queen of Scot |
| SETON | One of the ladies-in-waiting of Mary Queen of Scots (who collectively were known as the Four Marys) |
| JAMES | First name of the son of Mary, Queen of Scots who succeeded Elizabeth I (5) |
| ELIZABETHI | Henry VIII and Anne Boleyn's daughter whose reign as the last Tudor monarch saw the defeat of the Spanish Armada and execution of Mary, Queen of Scots (9,1) |
| STIRLING | Scottish city, site of a castle that was the childhood home of Mary, Queen of Scots (8) |
| ANTONIAFRASER | Historical biographer of Mary Queen of Scots and Oliver Cromwell, who also created Jemima Shore, the investigative journalist (7,6) |
| MARYSTUART | 1800 Friedrich Schiller verse play based on the life of Mary, Queen of Scots (4,6) |
