| BASRA | City with one of the world's oldest mosques |
| SALAMANCA | Spanish city with one of the world's oldest universities |
| ONEONTA | City with one of the SUNY schools |
| LAIDOFF | Got rid of American city with one to two parties (4,3) |
| CAMBRIDGE | English city, home to one of the world's oldest universities and a river where the Bumps races take place twice a year over several days (9) |
| ATHENS | One of the world's oldest cities and the largest city in Greece (6) |
| BORNEO | Home of one of the world's oldest rainforests |
| MET | Location of the world's oldest surviving piano, with "the" |
| DAMASCUS | One of the world's oldest cities, on the Barada River (8) |
| ALENCON | French city with one clan |
| AMATEUR | A friend goes to old city with one without expertise (7) |
| SOFIA | Foreign city with one installed in seat (5) |
| TWINNED | Matched or paired a city with one in a different country (7) |
| NOURISH | Support no old city with one shilling (7) |
| VETTURINI | Check out European city with one's cab drivers there? |
| ARCHERY | Featuring in films including Robin Hood, The Hunger Games and Brave, one of the world's oldest sports (7) |
| PARMA | Italian city famed for prosciutto and cheese, home to one of the world's oldest universities (5) |
| OLIVE | With stone fruits pressed for oil, preserved or used in Mediterranean and Provencal recipes including tapenade, one of the world's oldest known cultivated trees (5) |
| ARGOS | Greek city which is one of the world's oldest continuously inhabited cities (5) |
| IRAN | 17th largest country in area, with one of the world's oldest civilisations (4) |
