| EVANSVILLE | City in Indiana, US on the Ohio River home to Mesker Park Zoo and Botanic Garden (10) |
| OWL | Flaco the ___, bird that escaped the Central Park Zoo and is now living in Central Park |
| GARY | City in Indiana, US, founded in 1906 on the southern shore of Lake Michigan |
| CINCINNATI | City in SW Ohio, US, on the Ohio River (10) |
| PITTSBURGH | Pennsylvania city on the Ohio River (10) |
| TERREHAUTE | City in Indiana built on a site above the Wabash River |
| MADAGASCAR | Computer-animated 2005 DreamWorks film about animals from Central Park Zoo living in an island jungle |
| ANNOUNCERS | Newscasters in US on CNN are changing (10) |
| CIVICPRIDE | Lions at the L.A. or Central Park zoo |
| PADUCAH | City in Kentucky on the Ohio River at the mouth of the Tennessee River (7) |
| AGRA | Indian city in Uttar Pradesh on the Yamuna river, home to two UNESCO World Heritage Sites (4) |
| SOUTH | And 15 City in N Indiana, US, on St Joseph River associated with Studebaker automobiles (5,4) |
| ORINOCO | A river, home to the anaconda and to a species of crocodile which is South America's largest predato |
| BEND | City in N Indiana, US, on St Joseph River associated with Studebaker automobiles (5,4) |
| SOUTHBEND | City in N Indiana, US, on St Joseph River associated with Studebaker automobiles |
| AMAZON | South American river, home to the pink river dolphin (6) |
| WILSON | Sir Angus -; author of the story collection The Wrong Set and novels including The Old Men at the Zoo and No Laughing Matter (6) |
| URE | North Yorkshire river, home to brown trout, grayling, barbel, chub and roach (3) |
| PHYSIC | Chelsea - Garden; founded by the Worshipful Society of Apothecaries on land endowed by Hans Sloane, the oldest botanic garden in London (6) |
| FLACO | Owl that escaped from the Central Park Zoo in 2023 and became an online celebrity, before his death last month |
