| OLBIA | Ancient Roman city founded to defend the Tiber River (5) |
| SIENA | This Italian city was originally an Etruscan settlement that grew to become ancient Roman city. Its cathedral was begun in the 12th century in the Romanesque style but transformed in the 13th century |
| HURST | Castle built on Henry VIII's orders to defend the western approach to the Solent (5) |
| FORUM | Public square in an ancient Roman city (5) |
| OSTIA | Ancient Roman city port (5) |
| TUNIS | It was a Roman city on the north coast of Africa, near the site of ancient Carthage. It was occupied by the Germans in 1942 and liberated by Allied troops in 1943. In The Tempest (act 2, scene 1), Seb |
| GLAAD | Organization founded to combat homophobic reporting on the AIDS crisis |
| HYENA | "In all my associations with hunters, travellers and naturalists, I have never yet been able to find one who would defend the ____" (Theodore Roosevelt) |
| STING | Defend the hive, in a way |
| ACTUP | Group founded to combat AIDS |
| VATICANCITY | The seat of the Roman Catholic Church, and an enclave in Rome, situated on the west bank of the Tiber River. (7,4) |
| ICMSA | Which organisation defends the interests of Ireland's dairy farmers? (1,1,1,1,1) |
| MOAT | Split the atom to defend the castle (4) |
| ROME | Capital city on the Tiber River |
| CRIMINALLAWYERS | They're likely to defend the culprits and barman in The Young Offenders, for instance (8,7) |
| HOMEGUARD | British citizen army formed in 1940 to defend the UK against invasion, disbanded in 1957 (4,5) |
| ENTERPRISE | Star Trek's Starship used to defend the United Federation of Planets |
| HOLMES | Solver taking time off to defend the setter (6) |
| NERA | Tributary of the Tiber River in Italy |
| POMPEII | Ancient Roman city destroyed when Vesuvius erupted (7) |
