| MU | 12th letter of the Greek alphabet |
| ELL | How the 12th letter of the alphabet sounds |
| EL | Twelfth letter |
| NU | 13th letter of the Greek alphabet (2) |
| PI | 16th letter of the Greek alphabet (2) |
| XI | 14th letter of the Greek alphabet (2) |
| NI | Letter of the Greek alphabet |
| MUCKUP | Make a mess of Puck's anagram, putting the twelfth letter first (4,2) |
| LISTEN | Pay attention: the 12th letter is X |
| ELLE | Name that sounds like the 12th letter |
| IOTA | Ninth letter of the Greek alphabet, which gave rise to the ninth letter of the English alphabet (4) |
| DELTOID | In the human body, muscle named after the shape of the fourth letter of the Greek alphabet (7) |
| OMEGA | Twenty-fourth and final letter of the Greek alphabet, used as the symbol for the SI unit of electrical resistance, the ohm (5) |
| EPSILON | Fifth letter of the Greek alphabet; or, prefixed to "Carinae", the name of the star in Carina also called Avior (7) |
| THETAS | Several of the 8th letter of the Greek alphabet (6) |
| ALPHA | First letter of the Greek alphabet, as in the variety of meadow phlox (Phlox maculata) (5) |
| SIGMA | Eighteenth letter of the Greek alphabet, derived from the Phoenician letter shin (5) |
| DELTA | The fourth letter of the Greek alphabet lends its name to the ___ Force paintballing centre based in Worsley, Greater Manchester (5) |
| KAPPA | Tenth letter of the Greek alphabet, derived from the Phoenician letter kaph (5) |
| THETA | Eighth letter of the Greek alphabet, derived from the Phoenician letter teth (5) |