| MARYI | "Queen of Scots" monarch |
| GUISE | Mary of ---, queen consort of James V of Scotland and mother of Mary, Queen of Scots (5) |
| MARY | --- of Guise, queen consort of James V of Scotland and mother of Mary, Queen of Scots (4) |
| DARNLEY | Lord -; title of Henry Stuart, husband of Mary, Queen of Scots implicated in the murder of David Rizzio before being murdered himself at Kirk o' Field (7) |
| LEVEN | Loch ___, body of water in Perth and Kinross, Scotland housing a castle that was a place of imprisonment of Mary, Queen of Scots (5) |
| ELIZABETHI | Henry VIII and Anne Boleyn's daughter whose reign as the last Tudor monarch saw the defeat of the Spanish Armada and execution of Mary, Queen of Scots (9,1) |
| STUART | Henry -, Lord Darnley; second husband of Mary, Queen of Scots and the father of James I and VI of Scotland (6) |
| WALSINGHAM | Surname of Queen Elizabeth I's spymaster who secured the execution of Mary Queen of Scots (10) |
| ANTOINETTE | ___ of Bourbon, grandmother of Mary, Queen of Scots |
| SETON | One of the ladies-in-waiting of Mary Queen of Scots (who collectively were known as the Four Marys) |
| JAMES | First name of the son of Mary, Queen of Scots who succeeded Elizabeth I (5) |
| BOTHWELL | 4th Earl of -; title Mary, Queen of Scots husband who was implicated in the murder of Lord Darnley (8) |
| JAMESI | Son of Mary, Queen of Scots and king of England and Ireland from 1603 until his death in 1625 (5,1) |
| LOCH | Expanse of water in Scotland with seven islands, on one of which Mary Queen of Scots was imprisoned (4,5) |
| STIRLING | Scottish city, site of a castle that was the childhood home of Mary, Queen of Scots (8) |
| BABINGTON | When Mary, Queen of Scots wanted to assassinate Queen Elizabeth I, she began exchanging messages with her co-conspirator that used a mixture of codes and ciphers known as a nomenclator. The deciphered |
| LANGSIDE | Site of the last battle of forces loyal to Mary Queen of Scots before her exile to England (8) |
| LOCHLEVEN | Body of water in Scotland housing a castle that was the place of imprisonment of Mary, Queen of Scot |
| JAMESVI | Only child of Mary, Queen of Scots |
| ANTONIA | ---- Fraser, biographer of Mary Queen of Scots (7) |
